The QEW Highway runs right through the city which runs from Toronto to the Niagara Falls border crossing.
Greyhound and Coach Canada operate a bus service from the MTO building downtown located at 301 St. Paul Street. Frequent trips to Toronto, Niagara Falls, and beyond can be arranged.
GO transit train station located just off of St. Paul Street West, not far outside downtown. Most trains are scheduled for a couple of trips Friday, Saturday, and Sunday. There are services West to Hamilton, Burlington, Oakville, Toronto and beyond. There is service East to Niagara Falls. See gotransit.ca for more details.
There is also a GO bus pickup and drop off which may now be operating daily but the schedule varies. The stop is located at the corner of Dawsco Drive and YMCA Drive which is surrounded by Costco, PetsMart and the Fairview Mall. Services East to Niagara Falls or West to the Burlington Go Station for connecting trains. See gotransit.ca for more details.
Via Rail Canada and AMTRAK train station is located with the GO station not far outside of Downtown. Located on Ambrose Street, not far off of St. Paul West. There are services West to Toronto and Beyond and East for Niagara Falls and beyond into Buffalo USA for AMTRAK. Check viarail.ca or amtrak.com for more information.